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If you have a question or need a quotation for your project using one of our services, please just fill out the Inquiry Form on the "Contacts" page, and we will do our best to respond to your inquiry within 24 hours.
A free online tool for protein cleavage sites prediction with a chosen enzyme.
Calculate monoisotopic and average mass of a custom peptide or peptides predicted from a protein.
The cleavage specificities of selected enzymes.
A comprehensive and freely accessible resource of protein sequence and functional information.
Mass spectrum simulation tool for peptide/protein sequences. The number of charge can be custom defined.
An online tool predicts m/z values for SMS ions generated from selected fragmentation methods such as CID, ECD, or ETD. The ion type and maximum number of charge can be pre-defined.
PDB provides the most complete collection of information about the 3D structures of proteins, protein complexes and other biomolecules.
Useful links
HDX-MS Epitope Mapping Service
HDX-MS epitope mapping is a process that determines the antibody binding site on the target antigen by using Hydrogen/Deterium eXchange coulpled with Mass Spectrometry. At NovoAb we use the most advanced HDX-MS based analytical tools to provide accurate and reliable high-resolution epitope mapping service for our customers. Unlike epitope mapping using a peptide library, which only works the best for linear epitopes, our proprietary epitope mapping by HDX-MS technology - FineMapping works for both linear and conformational epitopes with significantly improved spatial resolution. The vast majority of antigen-antibody interactions rely upon binding to conformational epitopes, which are composed of amino acids that are discontinuous in the protein sequence but are brought together upon three-dimensional protein folding. This character has made conformational epitopes particularly difficult to map because they only exist in the native structure of a protein. Our fast HDX-MS epitope mapping service platform uses directly the intact antigen protein at its native conditions, which makes the technology well suited for mapping conformational epitopes. Read more about epitope mapping here.
Key features:
- 100% success rate
- Amino acid resolution
- Linear and conformational epitopes
- High-throughput screening of >50 candidates against one antigen
- Accurate mapping for glycosylated antigens by FineMapping technology
- Epitope mapping for unpurified/difficult targets
- Epitope mapping for bispecific antibodies
- Tailored service by top-down, bottom-up, or middle-down HDX-MS
- Unparalleled sensitivity using subzero HPLC technology
- Comprehensive and easy-to-understand report
High Resolution HDX-MS Service
Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) is a proven methodology for monitoring conformational aspects of proteins and biosimilars in solution. From protein conformational change to high-resolution higher-order structure (HOS) assessment, antibody epitope mapping and paratope mapping, and drug binding site determination, NovoAb offers tailored CRO services using individual or combined bottom-up HDX-MS, top-down HDX-MS, and the newest middle-down HDX-MS assays. We avoid the uncertainties caused by high level of back exchange through the use of subzero temperature UHPLC technologies. Read more here.
Key features:
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100% sequence coverage for mAb
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Minimized back exchange by subzero temperature LC-MS
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High resolution up to single-residue by SEED technology
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Tailored assay using bottom-up, top-down, or middle-down HDX-MS
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Enhanced coverage for glycosylated proteins
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Accurate protein binding site determination
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Higher-order structure comparison
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High resolution protein conformational change
Antibody & Biosimilar Characterization Service
From intact antibody molecular weight determination to drug conjugation site determination, fingerprint-like higher-order structure (HOS) comparative assessment, NovoAb provides a complete service package for antibody/ADC characterization and biosimilar testing. Our HDX-MS comparability data with 100% sequence coverage provides complete structural information that covers the whole antibody/biosimilar, without any missed regions or residues (read more about our technology here). This unique feature provides unparalleled confidence in assessing the higher-order structural similarity of different antibodies/ADCs. Our HOS assessments are performed according to FDA/EMA guidelines and our results have been used for regulatory filling application. No matter your sample is a small therapeutic protein or whole antibody/biosimilar or PEGylated biologics, we have a tailored solution to meet your needs. Read more about HOS characterization here.
Key features:
- Accurate MW assay for mAb, ADC, biosimilars, fusion proteins
- Complete peptide mapping
- Fast paratope mapping
- High-resolution higher-order structural (HOS) similarity assessment
- Receptor binding comparability assessment
- Precise drug conjugation site determination
- Disulfide mapping
- PTM profiling including glycosylation
- Minimized mg sample requirements
- Comprehensive report
BNAB Screening Service
Developing broadly neutralizing antibodies (bnAbs) against a virus and its mutant escapes is possible but only those who can bind to a cross-reactive epitope on the target antigen can do so. To quickly screen numerous antibody candidates against a cross-reactive epitope, NovoAb has developed the TASTE technology platform, which significantly reduces the turnaround time of our epitope mapping service. It is applicable to antigens such as Hemagglutinin of influenza, E protein of Zika, and Spike protein of the new coronavirus. We have successfully identified an effective cross-reactive epitope recently for SARS-Cov-2.
Key features:
- Fast & Cost-effective
- High-throughput
- Any antibody format (mAb, Fab, scFv, IgM, IgA etc.)
- Any viral protein target
- Works for linear and conformational epitopes
- Glycosylated epitopes
- Highly dynamic antigens
- Minimal sample requirements
- Free cross-reactive epitope identification
Vaccine Evaluation Service
The elicitation of effective neutralizing antibodies (nAbs) is a primary determinant of the efficacy of a new vaccine candidate. On the other hand, to develop a universal vaccine against various strains of a virus, it is critical to make sure the vaccine can induce antibodies targeting broadly neutralizing epitopes. To aid in the development of more effective vaccines, NovoAb's vaccine evaluation service platform can quickly characterize the binding epitope profile of vaccine induced antibody repertoires. It can also check if the desired antibodies directed toward a specific epitope (e.g. a broadly neutralizing epitope recognized by a bnAb) are present and provide epitope-targeted antibody response quantitation in biofluids such as serum. It is applicable to vaccines against any kind of viruses, including SARS-Cov-2 coronavirus, Influenza virus, Dengue virus and others.
Key features:
- Global epitope profiling for antibody repertoires
- Epitope-specific antibody induction evaluation
- Compare antibody induction for different vaccine formats
- Epitope-specific antibody response quantitation
- Vaccine optimization
- Universal vaccine development
- Epitope-focused immunogen design
- Epitope mapping for purified mAbs
- Broadly neutralizing antibody (bnAb) screening
- Comprehensive, high-throughput & cost-effective
