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A free online tool for protein cleavage sites prediction with a chosen enzyme.
Calculate monoisotopic and average mass of a custom peptide or peptides predicted from a protein.
The cleavage specificities of selected enzymes.
A comprehensive and freely accessible resource of protein sequence and functional information.
Mass spectrum simulation tool for peptide/protein sequences. The number of charge can be custom defined.
An online tool predicts m/z values for SMS ions generated from selected fragmentation methods such as CID, ECD, or ETD. The ion type and maximum number of charge can be pre-defined.
PDB provides the most complete collection of information about the 3D structures of proteins, protein complexes and other biomolecules.
Useful links
EMPEM polyclonal epitope mapping Service
Our Electron Microscopy Polyclonal Epitope Mapping (EMPEM) technology platform can quickly interrogate the immune responses against biologics. The EMPEM and cryo-EMPEM workflows allow you to study the polyclonal antibody responses against a biotherapeutics candidate. Antigen molecules bound with these antibodies can be directly isolated from the serum and subjected to high-resolution cryo-EM analysis. Detailed structural information allows the mapping of different epitopes on the biotherapeutics at single-residue and atomic resolution. The amino acid residues in these epitopes can then be engineered to reduce the immunogenicity for protein/antibody therapeutics and gene therapy viral vectors. EMPEM is also a powerful tool for mapping antibody responses to each epitope in the serum, providing immediate feedback for mechanistic evaluation of epitope-targeting. The abundance of pAbs to each epitope can also be calculated to show immune transitions over time.
For vaccine development, the elicitation of effective neutralizing antibodies (nAbs) is a primary determinant of the efficacy of a new vaccine candidate. To develop a universal vaccine against various strains of a virus, it is critical to make sure the vaccine can induce antibodies targeting broadly neutralizing epitopes. To aid in the development of more effective vaccines, NovoAb's EMPEM and cryo-EMPEM service platforms can quickly characterize the binding epitope profile of vaccine induced antibody repertoires. It can also check if the desired antibodies directed toward a specific epitope (e.g. a broadly neutralizing epitope recognized by a bnAb) are present and provide epitope-targeted antibody response quantitation in biofluids such as serum. It is applicable to vaccines against any kind of viruses, including SARS-Cov-2 coronavirus, Influenza virus, Dengue virus and others.
Key features:
- Minimal sample consumption
- Immunogenicity profiling
- Global epitope profiling
- Polyclonal antibody epitope mapping
- 3D high-resolution epitope mapping
- Compare antibody induction for different vaccine/biotherapeutics candidates
- Epitope-specific antibody response quantitation
- Broadly neutralizing antibody (bnAb) screening
- High-throughput & cost-effective
