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If you have a question or need a quotation for your project using one of our services, please just fill out the Inquiry Form on the "Contacts" page, and we will do our best to respond to your inquiry within 24 hours.
A free online tool for protein cleavage sites prediction with a chosen enzyme.
Calculate monoisotopic and average mass of a custom peptide or peptides predicted from a protein.
The cleavage specificities of selected enzymes.
A comprehensive and freely accessible resource of protein sequence and functional information.
Mass spectrum simulation tool for peptide/protein sequences. The number of charge can be custom defined.
An online tool predicts m/z values for MSMS ions generated from selected fragmentation methods such as CID, ECD, or ETD. The ion type and maximum number of charge can be pre-defined.
PDB provides the most complete collection of information about the 3D structures of proteins, protein complexes and other biomolecules.
Useful links
Epitope Mapping Service
Epitope mapping is a process that determines the antibody binding site on the target antigen. At NovoAb we use the most advanced HDX-MS and cryo-electron microscopy (cryo-EM) based analytical tools to provide high-resolution 3D epitope mapping service for our customers. Unlike epitope mapping using a peptide library, which only works for linear epitopes, our proprietary HDX-MS epitope mapping platforms works for both linear and conformational epitopes with single-residue resolution. On the other hand, our Rigidbody based cryo-EM mapping technology enables high-resolution 3D epitope mapping and paratope mapping for antibody-antigen complexes and binding site mapping for proteins, with single amino acid and near atomic resolution. The vast majority of antigen-antibody interactions rely upon binding to conformational epitopes, which are composed of amino acids that are discontinuous in the protein sequence but are brought together upon three-dimensional protein folding. This character has made conformational epitopes particularly difficult to map because they only exist in the native structure of a protein. Our fast HDX-MS epitope mapping service and cryo-EM epitope mapping service platforms use directly the intact antigen protein at its native conditions, thus are well suited for mapping conformational epitopes. Click and learn more about our HDX-MS epitope mapping service and cryo-EM service. Read more about epitope mapping here
HDX-MS Service
Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) is a proven methodology for monitoring conformational aspects of proteins and biosimilars in solution. From protein conformational change to high-resolution higher-order structure (HOS) assessment, antibody epitope mapping and paratope mapping, and drug binding site determination, NovoAb offers tailored CRO services using individual or combined bottom-up HDX-MS, top-down HDX-MS, and the newest middle-down HDX-MS assays. We avoid the uncertainties caused by high level of back exchange through the use of subzero temperature UHPLC technologies. Click and learn more about our HDX-MS service. Read more about HDX-MS here.
Cryo-Electron Microscopy (cryo-EM) Service
Our AbEM3D cryo-EM technology enables high-resolution 3D epitope mapping and paratope mapping for antibodies and binding site mapping for proteins and ligands, at single amino acid and atomic resolution. AbEM3D integrates modern biochemistry, automation, the most advanced hardware and software, and artificial intelligence (AI) powered image processing into a solution which can facilitate high-resolution structural characterization for even the most challenging targets, from very small proteins to large and complex protein assemblies. Samples are optimized, flash frozen in a wide range of naturally occurring conditions, and then used to collect thousands of cryo-EM images and build precise empirical 3D structural models. AbEM3D is also an excellent option for polyclonal antibody epitope mapping. Learn more about our cryo-EM service.
Antibody & Biosimilar Characterization Service
From intact antibody molecular weight determination to drug conjugation site determination, fingerprint-like higher-order structure (HOS) comparative assessment, NovoAb provides a complete service package for antibody/ADC characterization and biosimilar testing. Our HDX-MS comparability data with 100% sequence coverage provides complete structural information that covers the whole antibody/biosimilar, without any missed regions or residues (read more about our technology here). This unique feature provides unparalleled confidence in assessing the higher-order structural similarity of different antibodies/ADCs. Our HOS assessments are performed according to FDA/EMA guidelines and our results have been used for regulatory filling application. No matter your sample is a small therapeutic protein or whole antibody/biosimilar or PEGylated biologics, we have a tailored solution to meet your needs. Click and learn more about our antibody biosimilar characterization service. Read more about HOS characterization here.
Antibody Screening Service
Developing broadly neutralizing antibodies (bnAbs) against a virus and its mutant escapes is possible but only those who can bind to a cross-reactive epitope on the target antigen can do so. To quickly screen numerous antibody candidates against a cross-reactive epitope, NovoAb has developed the TASTE technology platform, which significantly reduces the turnaround time of our epitope mapping service. It is applicable to antigens such as Hemagglutinin of influenza, E protein of Zika, and Spike protein of the new coronavirus. We have successfully identified an effective cross-reactive epitope recently for SARS-Cov-2. Learn more about our antibody screening service here.
EMPEM polyclonal epitope mapping Service
Our EMPEM (Electron Microscopy Polyclonal Epitope Mapping) and cryo-EMPEM technology platforms are excellent options for interrogating the immune responses against biologics such as biotherapeutics and vaccines. The EMPEM workflow allows you to study the polyclonal antibody responses against an immunogen/vaccine candidate. Antigen bound to these antibodies can directly be isolated from the serum and subjected to high-resolution cryo-EM analysis. Detailed structural information allows the mapping of different epitopes on the vaccine/biotherapeutics. These epitopes can then be engineered to enhance the immunogenicity for vaccine design and reduce the immunogenicity for protein/antibody therapeutics and gene therapy viral vectors. EMPEM and cryo-EMPEM are also powerful tools for mapping antibody responses to each epitope in the serum, providing immediate feedback for mechanistic evaluation of epitope-targeting in vaccines. The abundance of pAbs to each epitope can also be calculated to show immune transitions over time. Learn more about our EMPEM service here.
